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Guaiacol peroxidase (GP) activities were estimated as oxid ation of guaiacol to the formation of tetraguaiacol (ε =26.6 mM-1 cm-1) in 3 ml reaction mixtures containing 0.95 ml of citrate buffer (100 mM, pH 4.6), 0.95 ml of H 2O, 1 ml of guaiacol 15 mM, 50 µl of H 2O2 32 mM, 50 µl enzyme extract (Liu et al., 1999). The peroxidase, and superoxide dismutase (SOD), all of which deal with the destruction ofpotentially toxic reduced forms ofoxygen(11). Wefoundthatall speciescontainedSOD,but peroxidase and catalase were not uniformly distributed amongthe species. L.pnelumophila andLegionellagormanii had only peroxidase, and L. dmnoffli had both peroxidase and catalase.

Guaiacol peroxidase

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Titration of guaiacol by hydrogen peroxide in the presence of a catalytic amount of horseradish peroxidase shows that the reduction of hydrogen peroxide proceeds by the abstraction of two electrons from a guaiacol molecule. In the same way, it can be demonstrated that 0.5 mol of guaiacol can reduce, … uthje, S. (2003) Properties of guaiacol peroxidase activ-ities isolated from corn root plasma membranes. Plant Physi ol. 132, 1489–1498.

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(Brassica napus var. okapi) Root During Different Growth Stages.

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Determining the average level of peroxidase in different cultivars can help guide breeding decisions. We have investigated the lignin peroxidase-catalyzed oxidation of guaiacol and the role of veratryl alcohol in this reaction by steady-state and pre-steady-state methods. Pre-steady-state kinetic analyses demonstrated that guaiacol is a good substrate for both compounds I and II, the two- and one-electron oxidized enzyme intermediates, respectively, of lignin peroxidase. The rate constant for The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substituent effects can modulate enzyme activity in DHP, we investigated its Key words: Safflower, guaiacol peroxidase, kinetics, isoenzymes, salt stress.

Guaiacol peroxidase

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P8125, P8170, P8250, P8375, P8415, and P8651. The activi ty of ascorbate peroxidaseand guaiacolperoxidaseincreased after 3-6 hours af- ter the hardening and reached its maximum after 24 hours, when there was the most significant in- crease in heat resistance of seedlings. Total guaiacol peroxidase activities from soluble and microsomal fractions of maize roots were compared and showed weak changes. By contrast, total plasma membrane and washed plasma membrane peroxidase activities, representing peripheral and integral membrane proteins, revealed strong changes after all of the stresses applied. Peroxidase is an enzyme that can catalyze the cross-linking of phenolic residues by H2O2 resulting in undesirable color changes (graying) and the potential generation of off-flavors in a food product.
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Guaiacol peroxidase

A significant increase in the superoxide dismutase activity in the leaves of BZ was observed under  proline, protein and carotenoid contents, and catalase (CAT), ascorbate peroxidase (APX), Guaiacol peroxidase (GPX) and glutathione reductase (GR) activity  transparent to slightly yellow Guaiacol is transformed into the clearly-visible, brownish complex Tetraguaiacol with the enzyme peroxidase from the Detection  In contrast to the alternative guaiacol method, Peroxtesmo MI is odourless and non-toxic.Portabla testkit för snabba och enkla miljöanalyser i jord, vatten eller  Extensinperoxidase | Guaiacol peroxidase | Guaiacolase | HRP 77333 | Heme peroxidase | Lactoperoxidase | MPO | Manganese-dependent peroxidase  108010001336 Horseradish Peroxidase Proteins 0.000 claims description 3 description 1; 229930009858 Guaiacol Natural products 0.000 description 1  RBBR, methyl green and guaiacol as an indicator for the production of lignin laccase but no manganese peroxidase activity was detected.

Greiner Bio One   of ascorbate peroxidase, catalase and guaiacol peroxidase. A significant increase in the superoxide dismutase activity in the leaves of BZ was observed under  proline, protein and carotenoid contents, and catalase (CAT), ascorbate peroxidase (APX), Guaiacol peroxidase (GPX) and glutathione reductase (GR) activity  transparent to slightly yellow Guaiacol is transformed into the clearly-visible, brownish complex Tetraguaiacol with the enzyme peroxidase from the Detection  In contrast to the alternative guaiacol method, Peroxtesmo MI is odourless and non-toxic.Portabla testkit för snabba och enkla miljöanalyser i jord, vatten eller  Extensinperoxidase | Guaiacol peroxidase | Guaiacolase | HRP 77333 | Heme peroxidase | Lactoperoxidase | MPO | Manganese-dependent peroxidase  108010001336 Horseradish Peroxidase Proteins 0.000 claims description 3 description 1; 229930009858 Guaiacol Natural products 0.000 description 1  RBBR, methyl green and guaiacol as an indicator for the production of lignin laccase but no manganese peroxidase activity was detected.
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Total guaiacol peroxidase activities from soluble and microsomal fractions of maize roots were compared and showed weak changes. By contrast, total plasma membrane and washed plasma membrane peroxidase activities, representing peripheral and integral membrane proteins, revealed strong changes after all of the stresses applied. oxygen, and the oxygen reacts with the Guaiacol to produce a brown product, oxidized Guaiacol.